Exosomal RNA Sequencing

Overview:

Exosomal RNA sequencing (ExoRNA-Seq) is used to study the RNA molecules found in exosomes which are membrane-bound extracellular vesicles released by most eukaryotic cells into body fluids like blood, urine, saliva, and others. Exosomal RNAs include mRNA, miRNAs, long non-coding RNAs (lncRNAs), and small RNAs. Exosomal RNAs attract more and more attention due to its stability in body fluids and its potential applications in biomarker discovery, disease diagnosis, and understanding intercellular communication.

Applications:

Provide a snapshot of tumor-specific changes in gene expression. Exosomal RNA (particularly miRNAs and mRNAs) can be used as biomarkers for early cancer detection, identifying tumors, monitoring metastasis, and assessing treatment response.
Detect and monitor the levels of disease-related exosomal RNAs that could serve as biomarkers or therapeutic targets in neurodegenerative diseases like Alzheimer’s and Parkinson’s.
Detect and monitor the levels of disease-related exosomal RNAs that could reflect pathological changes in Cardiovascular diseases.
Used to study cells response to infections and immune responses and pathogen-host interactions in infectious diseases.